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The Muscular System (Ch. 10)
(terminology: myo, mys = muscle)
- Characteristics of muscle tissue
- Excitability - can receive and respond to a stimulus (electrical)
- Contractility - individual cells contract (shorten) when stimulus is received; convert
chemical energy (ATP) to mechanical energy (movement)
- Extensibility - can be stretched
- Elasticity - return to original shape after contraction or extension
- Review 3 types of muscle tissue from Muscle Table (in tissue notes): including shape of
fibers (cells), locations, etc.
- Skeletal muscles - attach to skeleton; tissue making them up is skeletal muscle tissue, which
is striated & voluntary
- Functions of (skeletal) muscular system
- Motion
- Posture
- Support
- Guards openings
- Heat production
- Stability of joints
- Keeps fluids (blood & lymph) moving
- Organization of a skeletal muscle (fig. 10. __)
- Connective tissue component - binds muscle fibers together into a bundle called
a muscle, and anchors muscle in place
- Whole muscle wrapped in epimysium - sheet of dense irregular
collagenous connective tissue
- Perimysium (extensions of epimysium) - penetrates muscle and divides it
into fascicles (visible muscle 'strings')
- Endomysium (extensions of perimysium) - penetrates fascicle and
separates it into muscle fibers (cells)
- Epi-, peri-, and, endo-mysium are continuous w/ one another
- Epimysium extends beyond muscle to become - tendons or aponeuroses
- Tendons & aponeuroses are continuous w/ periosteum of bone or
epimysium of another muscle
- Nerve and blood supply
- Skeletal muscle fiber contracts only when - stimulated by a neuron from
the somatic (voluntary) division of the nervous system
- Muscle fibers need good blood supply - demand lots of nutrients and
oxygen for contraction
- Nerves & blood vessels travel through the connective tissue to deep in
the interior of muscle
- each muscle fiber makes contact with one neuron in one spot
- each muscle fiber is surrounded by blood capillaries
- Histology (fig. 10.2, 10.3, 10.6)
- Muscle fibers (recall muscle cell = muscle fiber)
- A muscle fiber is microscopic (10-100 micrometers) in diameter; but up to
12 inches in length
Components of a muscle fiber:
- Sarcolemma = cell membrane in skeletal muscle cells
T tubules - invaginations of sarcolemma that extend into muscle
fiber
- Sarcoplasm = cytoplasm in skeletal muscle cells
- single fiber has many nuclei in sarcoplasm
- stores glycogen (how animals store glucose)
- has many mitochondria (why?)
- has myoglobin (protein similar to hemoglobin) - binds to and stores
a small amount of oxygen; makes muscle red
- sarcoplasmic reticulum = smooth ER in skeletal muscle cells;
system of membrane tubes that wrap internal cellular structures;
packed with calcium
- myofibrils - rod- shaped submicroscopic (1-2 micrometers in
diameter) protein structures; run throughout the long axis of a
muscle fiber
- Organization of a skeletal muscle (recap; fig 10.6)
- Skeletal muscle - surrounded by epimysium; contains many muscle
fascicles
- Muscle fascicle - surrounded by perimysium; contains many muscle fibers
(cells)
- Muscle fiber - surrounded by endomysium; contains many myofibrils
- Myofibrils - surrounded by sarcoplasmic reticulum; contain two kinds of
myofilaments (short, thread-like protein structures) organized into
sarcomeres (fig. 10.5, 10.7)
Components of a sarcomere:
- thin myofilaments
- made mostly of actin protein
- shaped like a thin filament with loops (handles) sticking out
- also have two other associated proteins: troponin,
tropomyosin
- thick myofilaments
- made of myosin protein
- shaped like a rod w/ heads (hands) sticking out
- hands sometimes grab actin handles
- the arrangement of these proteins (sarcomere) gives skeletal
muscle its striated appearance: thin filaments on the outside, thick
filaments on the inside
- Sarcomere structure (fig. 10.4, 10.5)
- Ends of a single sarcomere are Z-lines
- Actin (thin) filaments attach to Z-lines and extend toward center of
sarcomere, but do not touch one another
- Myosin (thick) filaments 'float' in middle of sarcomere
- Pattern:
- A-band = region in which actin and myosin overlap (dark)
- I-band = region which has actin thin filaments only (light)
- H-zone = region which has myosin thick filaments only (in center
of each sarcomere)
- NOTE: A-bands and I-bands (respectively) are the visible dark and
light striations
- A chain of sarcomeres makes up a myofibril (think 'boxcars on a train')
- Skeletal muscle contraction - the sliding filament theory
- Recall
- Sarcomere is the functional (contractile) unit of muscle tissue
- Thin (actin) filaments run from the middle of the sarcomere, and are
attached to the Z-lines; each actin filament has active sites ("handles")
- In between actin filaments are myosin (thick) filaments, which don't touch
the Z-line; each thick filament has heads ("hands")
- How a sarcomere contracts:
- To contract (shorten) sarcomere (and therefore shorten muscle fiber),
myosin "hands" must grab actin "handles", and pull
- This will shorten the whole sarcomere
- When an active site "handle" and a head "hand" are attached, it is called
a crossbridge
- ATP is required to for the "hand" to let go of the "handle"
- Hand grabs the next handle, etc.
- Notes
- Length of sarcomere shortens, but
- Length of myofilaments does not change
- H-zone and I-band may disappear during contraction (fig. 10.8)
- Many sarcomeres contracting together shortens an entire myofibril, which will
shorten the whole muscle fiber, which will shorten the whole muscle
- Control of skeletal muscle activity; How is muscle contraction triggered?
- Motor unit = one neuron + the skeletal muscle fiber(s) it innervates (fig. 10.9)
- Muscle fiber must have stimulus to contract
- Nerves (specifically, axons of motor neurons) attach directly to surface
(sarcolemma) of muscle at neuromuscular junction
- Excitation-contraction coupling (table 10.1):
Action potential (AP) on axon Ca+2 channels open acetylcholine secreted
from axon (via exocytosis) acetylcholine hits receptors on sarcolemma local
AP generated on sarcolemma AP transmitted through T tubules AP reaches
sarcoplasmic reticulum
- Contraction cycle begins: (fig. 10.10)
- Release of calcium from SR: AP causes calcium to be released from
the ER of the muscle fiber (sarcoplasmic reticulum) into the sarcoplasm
(cytoplasm)
- Exposure of active sites: calcium allows muscle contraction to occur by
exposing the actin active sites "handles"
- NOTE: Under normal circumstances, the actin active sites
"handles" are covered with a protein called tropomyosin
(barricade), which prevents crossbridges from forming
- When calcium is present, it "turns off" the tropomyosin by binding
to it at small fibers called troponin (on/off switch) fibers
- Crossbridge attachment: now the actin "handles" are available for the
myosin "hands" to form a crossbridge
- Pivoting of myosin head: hands pull (powerstroke)
- Crossbridge detachment and myosin reactivation: hands release and
recock for the next powerstroke (requires ATP)
- NOTES:
- Attachment, movement, and release happens many times during a single
contraction
- When calcium in cytoplasm returns to normal levels, contraction stops
- Relaxation:
- NOTE: acetylcholine is released and is almost simultaneously
destroyed by an enzyme present at the neuromuscular junction
(acetylcholinesterase), so that the fiber will not be continuously
stimulated
- AP on axon ends
- acetylcholine destroyed
- AP on sarcolemma stops
- Ca+2 is actively transported into SR for storage
- with Ca+2 removed, barrier is up; (tropomyosin) prohibits
crossbridge formation
- actin (thin) filaments slip back into resting position
- stays relaxed until another AP arrives
- This process produces lots of heat! *shiver
- Clinical notes
- Rigor mortis
- No ATP being made after death, so ...
- Calcium can't get back into SR
- Myosin "hand" can't detach from actin "handle"
- Myosin hand also can't re-cock
- Causes sustained muscle contraction - stiff as a board
- Stiffness leaves body 12-15 hours after death, as cells begin to
decompose
- Myasthenia gravis - autoimmune disease that causes destruction of
motor end plates; lose Ach receptors
- Botulism - often contracted from improperly canned foods; toxin produced
by bacterium prevents release of Ach; paralysis
- Chemicals which affect neuromuscular junction
- curare - blocks receptors on sarcolemma; causes asphyxiation b/c
of paralysis of diaphragm
- some nerve gases and pesticides - inhibit acetylcholinesterase;
effect?
- Energy for contraction (table 10.2 and fig. 10.18)
- ATP is source
- Obtaining ATP from food is slow; several reserves exist
- ATP stored in thick myofilaments (2 seconds)
- Creatine phosphate + ADP creatine + ATP (15 seconds)
Enzyme needed is CPK (creatine phosphokinase); CPK spills out of
damaged muscle cells; can be used diagnostically
- Break down glycogen
glycogen (hydrolysis) glucose (glycolysis) pyruvate (fermentation) lactic acid +
ATP
Krebs cycle and electron transport system
(requires O2)
CO2 + H2O + lots of ATP
- Finally, fatigue due to lack of ATP and buildup of lactic acid
- 'Oxygen debt' built up; heavy breathing reverses fermentation and sends
it down Krebs cycle and ETS; ATP replaced
- Of energy released as glucose is broken down, only about 40% is used
for muscle contraction; rest released as heat
- All-or-none law of skeletal muscle contraction
- A muscle fiber either contracts to its fullest extent or does not contract at all
"Fullest extent" may be modified by fatigue (lack of nutrients or accumulation of
wastes)
- How is amount of tension in a muscle controlled?
- Motor unit summation
- Recruitment = how many fibers contract; determines the degree of
muscle contraction
- The # of muscle fibers contracting depends on the number of motor
neurons firing
- The number of motor neurons firing is determined by the central
nervous system
- Muscles are divided into motor units (groups of muscle fibers
working together)
- Precise movements - 10 muscle fibers innervated by one
neuron
- Gross movements - 500 muscle fibers innervated by one
neuron
- In order to increase the flex of a muscle, more motor units are
recruited = motor unit summation
- Temporal summation
- a muscle is not allowed time to completely relax between
contractions
- the first contraction and the second contraction are added to one
another
- Kinds of contraction (depends upon kind of stimulus) REVIEW ON YOUR OWN
- Twitch (fig. 10.10)
- Rapid, jerky response to a single stimulus
- Does not normally occur in body
- Spasmodic twitching made involuntarily by muscles usually under
voluntary control = tic
- Incomplete tetanus (fig. 10.13b)
- Stimuli come so rapidly that the muscle can only partially relax b/w stimuli
- Results in sustained contraction
- Complete tetanus (fig. 10.13c)
- Stimuli come faster still
- No relaxation at all
- Other terms
- Tetanus
- an infectious disease with intermittent spasms and convulsions
- toxin from bacteria growing in dirty wound alters the transmission
of the nerve impulse to the muscle fibers
- sometimes called 'lockjaw'
- Spasm - sudden, involuntary contraction of short duration
- Cramp - painful, involuntary, complete tetanic contractions of a group of
muscles
- Charleyhorse
- result of injury; inflammation of muscles and connective tissue in
thigh resulting in pain & muscle spasms
- same condition in lower back = lumbago
- Tonic contraction (tonus or tone)
- Sustained, partial contraction of portions of muscle in response to stretch
receptors
- At any given time, some fibers are contracting, while others are relaxed.
Tightens muscle, but doesn't produce movement. Even relaxed muscles
have tone.
- Motor units alternate firing, and relieve each other. Tonus can be
maintained for a long time
- Essential for maintaining posture
- A muscle w/ less than normal tone = flaccid
- damage to nerve - muscle eventually replaced w/ connective
tissue; irreversible
- disuse atrophy - bedridden or cast; can be reversed after short
durations (4 months); lose muscle after 2 years
- Muscle growth
- In embryo, mitosis occurs forms many small (uninucleated) muscle cells
cells fuse to form long, multinucleated muscle fibers (syncytium) (fig. 10.2)
- Once the cells have fused, fibers cannot undergo mitosis
- At birth, child has all the muscle fibers he will ever have
- Increase in size of muscle results from increasing length and diameter, not from
more fibers
- In adult, muscles hypertrophy only if contracted to 75% maximum tension
- only a few contractions at a time are necessary
- work out every other day; muscles grow during rest and recovery
- increases # of myofibrils and myofilaments, not # of fibers
- mostly white fibers (fast-twitch) that enlarge (little myoglobin, make
ATP anaerobically, but large and strong) fig. 10.19b
- Weak activity (<75% max tension), even if prolonged, will not result in
hypertrophy
- red fibers (slow-twitch) respond (make ATP aerobically) fig. 10.19a
- develop more capillaries, more myoglobin, more mitochondria
- results in greater endurance; fatigue resistant, if oxygen available
- Repair of skeletal muscle (and cardiac muscle) is usually with scar tissue
(process = ___________________)
- Satellite cells
- Cardiac muscle tissue (fig. 10.21)
- Main component of heart wall
- Striated but involuntary
- Similarities to skeletal muscle tissue
- Arrangement of actin and myosin
- Sarcoplasmic reticulum
- T-tubules
- Differences
- Shorter fibers w/ one nucleus per fiber
- Branched fibers which join w/ one another to form network
- Thickening of sarcolemma b/w ends of fibers (intercalated disks). Disks have:
- Desmosomes which add strength to heart muscle
- Gap junctions through which ions pass from cell to cell; allows direct
transmission of AP through entire network of muscle fibers
- Energy for contraction is produced almost entirely aerobically (needs
constant supply of oxygen)
- Two networks
- Upper chambers (_____________) form a network
- Lower chambers (_____________) form a network
- One fiber in one network stimulated, stimulus spreads, and whole network
contracts together
- Source of stimulation
- Each skeletal muscle fiber must receive an impulse to contract
- Cardiac muscle fibers can contract w/o a nerve impulse; depolarize
spontaneously
- Lump of specialized muscle tissue in heart wall (sinoatrial - SA - node)
- 72X/minute, SA node sends out an electrical impulse that spreads over
the network and stimulates contraction
- Autonomic (involuntary) nerves to heart can speed up this rate of
contraction or decrease it
- Smooth muscle tissue (fig. 10.22)
- Nonstriated and involuntary
- Cells small, spindle-shaped, uninucleated
- Contain actin and myosin, but filaments are not so orderly (no sarcomeres, no
striations)
- Can contract well even when stretched (plasticity)
- Types
- Visceral (single-unit)
- Walls of hollow visceral organs (stomach, intestines, bladder, uterus)
- Cells bound together in sheets; contract as a unit
- tends to depolarize spontaneously; affected by hormones,
stretching, etc.
- gap junctions allow AP to move from cell to cell
- AP moves slowly; contraction occurs in waves (peristalsis)
- Multiunit
- Walls of blood vessels, walls of airways, around pupil, arrector pili
- Each cell has its own nerve supply, from the autonomic (involuntary)
division of the nervous system; impulse does not spread from cell to cell
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